Influence of castration methods on the subcutaneous auto - transplantation of mouse ovaria
LI Yu-bin
MAI Qing-yun
LI Tao
ZHOU Can-quan
Abstract：Objective To investigate the influence of two castration methods on subcutaneous auto - transplanta-tion of mouse ovarian tissue in SCID mice. Methods According to the operation methods,the mice were divided into three groups:control group,abdominal castration group and backside castration group(n = 10). The operation time and mouse survival rates were recorded after operation. Ovarian stimulation was carried out in all 3 groups 2 weeks later,after which follicle survival rate was recorded. Results There were 3 deaths after operations in the abdominal castration group, while no death was revealed in the other two groups. Significantly lengthened operation time was observed in abdominal castration group than backside castration group[(18. 6 ± 2. 7)min vs(12. 5 ± 3. 1)min,P < 0. 05]. The follicle surviv-al rates in the two castration groups were significantly less than control group(P < 0. 05),while there was no significant difference between the two castration groups(P > 0. 05). Conclusion Backside castration combined subcutaneous auto- transplantation of ovarian tissue was simpler than the abdominal one,with similar follicle survival rates.
Keywords：ovarian tissuesubcutaneous auto - transplantationcastration
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1647-1649 )