Effects of Safflower injection on the expressions of AKT in human hepatoma HepG -2 cells
Abstract：Objective To investigate the effect and mechanism of Safflower injection on cell proliferation of human hepatoma cells HepG-2 in vitro.Methods HepG-2 cells were treated by different concentrations (0, 50, 150, 250 g/L) of safflower injection .Fluorescence microscopy was applied to test the morphological changes of apoptosis by AnnexinⅤ-FITC/PI staining.Quantitative real-time PCR was carried to assess the mRNA expression of AKT.Western blot were used to observe the protein expression levels of AKT and p -AKT in HepG-2 cells.Results After treated with Safflower injection , HepG-2 cells presented the typical modality of early apoptosis; with significantly dose -dependent down-regulation in AKT mRNA, and AKT and p-AKT proteins.Conclusion Safflower injection inhibits cell prolifera-tion and induces apoptosis in HepG -2 cells through AKT signaling pathway .
Keywords：safflower injectionHepG-2cell proliferationcell apoptosisAKT
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1487-1490 )