Effects of Peroxiredoxin-1 on proliferation and JNK pathway of pulmonary fibroblasts of rats

Abstract：Objective To investigate the effects of Peroxiredoxin -1 (Prx-1) on proliferation and JNK pathway of pulmonary fibroblasts ( FB) of rats.Methods Cultured FB were randomly divided into four groups , control:0.4%fe-tal bovine serum;TGF-β1:final concentration is 5μg/L;TGF-β1 +vehicle:the empty plasmid was transected into FB for 36 h and then cells were treated with TGF -β1 (5 μg/L);TGF-β1 +Prx-1 plasmid:the plasmid including Prx-1 gene was transected into FB for 36 h and then cells were treated with TGF -β1(5μg/L).The plasmid was transfected in-to FB using the liposome infection .Immunofluorescence was used to evaluate plasmid transfection and 8-OhdG levels in FB;while MTT assay was used to evaluate FB proliferation and western blot was used to evaluate the expressions of phos -phorylated JNK ( p-JNK) , total JNK and Prx-1.Results The plasmids were successfully transfected into FB , showing as green florescence in cytoplasm and increased expression of Prx -1 protein.TGF-β1 significantly stimulated FB prolif-eration, and up-regulated the expression of p -JNK and 8 -OhdG in FB, which were markedly inhibited by Prx-1 plasmid transfection but not vehicle .Conclusion TGF -β1 induces FB to generate reactive oxygen species ( ROS ) , which contributes to JNK activation and FB proliferation;however , Prx-1 overexpression inhibits these changes by reduc-ing ROS level.