Effects of 15-deoxy-△12,14 -prostagliandxin J2 on hypertrophic scar in rabbit ears

ZHAO Xiao-kai; PAN Shu; XIE You-fu; FANG Li; LIANG Pei-hong; DAI Li-bing

Abstract：Objective To investigate the effect of 15-deoxy-△12,14 -prostagliandxin J2 (15d-PGJ2) on the expression of CD34 and TGF-β1 on hypertrophic scar in rabbit ears , and the possibility of 15d-PGJ2 in treatment of hy-pertrophic scar .Methods Ear hypertrophic scar model was established in 9 New Zealand white rabbits .Four wounds with total skin loses (1 cm ×1 cm) on the ventral side on each ear were constructed in all 9 rabbits.The wounds were randomly divided into the 15d-PGJ2 treatment group and control group , in which 10 μL 15d-PGJ2 and normal saline, respectively, were injected into the ear scar once a day for 7 days.On the 3rd, 6th and 17th day after the injection, 12 scars of each group were harvested .The expression of CD34 and TGF -β1 was detected by immunohistochemical method . Results Excessive dermal scars on rabbit ear that were similar to human hypertrophic scar were observed in the both two groups.Compared with control group , smaller, softer, flatter and color lighter scars were observed in treatment group .The expression of CD34 and TGF-β1 in the 15d-PGJ2 group was significantly down -regulated as compared with that in control group at different time points (P<0.05).Conclusion The 15d-PGJ2, ligand of PPAR-γ, can reduce the ex-pression of CD34 and TGF-β1 of hypertrophic scar in the rabbit ears and plays an important role in the prevention and treatment of hypertrophic scar .