The role of excision repair gene XPD in regulation of P53 and Ets-1 in hepatoma cells
TIAN Ming
YOU Chun-jiao
ZHANG Ji-xiang
Abstract：Objective To investigate the effects of xeroderma pigmentosum D ( XPD) on the growth of hepatoma cells and the expressions of P53 and Ets-1.Methods Human hepatoma cells (HepG2) were transfected with the plas-mids of pEGFP-N2-XPD using Lipofectamine 2000, and subsequently incubated with 20μmol/L Pifithrin-α( P53 in-hibitor) for 24 h.Blank control group (no transfecting and adding medicine ) was set.The expressions of XPD, P53, phospho-P53 (ser-15) and Ets-1 were assessed by RT-PCR and Western blot.Flow cytometry was used to examine the cell cycle and apoptosis .MTT assay was used to detect the cell proliferation .Results After transfected with the pEG-FP-N2-XPD plasmid, the mRNA and protein expression of XPD and P53 were significantly up -regulated, while the expression of Ets-1 was significantly down -regulated (P<0.01).Moreover, the cell proliferation was inhibited and the apoptosis was accelerated with pEGFP -N2-XPD plasmid transfected , which was reversed by Pifithrin -α.Conclusion Wild-XPD gene can restrain the proliferation, down -regulate Ets -1 via P53 pathway, and impel the apoptosis ofhepatoma cells.
Keywords：liver neoplasmsxeroderma pigmentosum group D genetransfectionP53 geneproto-oncogene protein Ets-1
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1149-1153 )