Tissue engineering of cardiovascular tissue using modified static ceeding method
YANG Chao
CHI Fan-wu
DU Jing
ZHOU Jun
PENG Bin
WANG Wei
WENG Ding-guo
Abstract：Objective To evaluate the application of modified static cell seeding method in tissue engineering of 2-dimensional cardiovascular patch .Methods Patch scaffolds fabricated from poly -4 -hydroxy -butyrate ( P4HB) polymers were seeded with human umbilical artery myofibroblasts ( HUAMs) using static cell seeding method .In static cultivation group , the cell-polymer patch constructs were cultured in vitro for 21 days under static cell culture condition . In dynamic group , the cell-seeded patch constructs were incubated in vitro for 14 days under static culture conditions and were subsequently transferred into a bioreactor for another culture of 7 days.Morphological characterization of tissue engi-neered ( TE) patches was observed included histology and scanning electron microscopy ( SEM ) .Extracellular matrix ( collagen ) formation was analyzed by immunohistochemistry .Results After static cell seeding and subsequently condi-tioning in the bioreactor , the fibroblast cells were well adhered to the surface of P 4HB polymer, the cells were mostly via-ble, had grown into the pores , and had formed uniform tissue on the patch constructs .Conclusion Using modified static cell seeding method , the uniform distribution of cells and tissue on the 2-dimensional tissue engineered structures can be achieved.
Keywords：tissue engineeringcardiovascular patchcell seedinghuman umbilical artery myofibroblasts
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1146-1148 )