Effects of chymase on proliferation of cultured dog vascular smooth muscle cells
SUN Cheng-lin
MA Jun
LIU Zong-feng
BAI Ying-kui
ZHAO Yan-peng
ZHANG Shao-jun
Abstract：Objective To investigate the effect of chymase on the cell proliferation and angiotensin Ⅱ( AngⅡ) generation of dog vascular smooth muscle cells .Methods The dog artery smooth muscle cells were cultured and divided into four groups:Control group, Angiotensin Ⅰ(Ang Ⅰ) group, Captopril group and Chymostatin group .Cell counting was performed by trypan blues staining , while the ratio of light density by MTT and Ang Ⅱconcentration were measured in the culture medium .Results The cell counts were significantly reduced in Captopril group and Chymostatin group com -pared with Ang Ⅰgroup (P<0.05).The cell proliferation of Chymostatin group was significantly inhibited (P<0.05). Moreover, the concentration of Ang Ⅱin Chymostatin group was reduced significantly (P<0.05).Conclusion Chymase may play an important role in the generation of Ang Ⅱand the proliferation of cultured dog vascular smooth muscle cells , via which chymostatin may inhibit cell proliferation .
Keywords：chymasedog smooth muscle cellangiotensin Ⅱcell proliferation
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1143-1145 )