The role of Ero1-Lαin hypertension-induced vascular endothelium injury
WANG Li-yao
LU Jing
ZHANG Jian-zhong
DONG Jian-da
CHAO Xiang-mei
GUO Feng-ying
HE Mao-tao
JING Li
Abstract：Objective To investigate the role of endoplasmic oxidoreductin -1-like protein alpha ( Ero1-Lα) in hypertension-induced vein endothelium injury , and further to confirm the vital role of endoplasmic reticulum ( ER) stress in the development of hypertension .Methods Human umbilical vein endothelial cells ( HUVECs) were cultured in hypertension mimic status via culturing with different concentrations of angiotensin Ⅱ( AngⅡ) .MTT was used to investi-gate the apoptosis rate of HUVECs , while Western blot and immunofluorescence histochemistry were applied to assess the expression of Ero1-Lα.Results The apoptosis of HUVECs was observed 24 h after stimulus of Ang Ⅱ.With the AngⅡconcentration rising , increase in the apoptosis rate of HUVECs , and Western blot expression and average immunofluo-rescence optical density of Ero 1-Lαwere revealed .Conclusion Ang Ⅱ can induce apoptosis of HUVECs , in which high expression of Ero 1-Lαparticipates .
Keywords：angiotensin Ⅱendothelial cells injuryEro1-Lα
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 824-827 )