The effects of different thawing methods on cardiac valves cryopreserved in liquid nitrogen with chondroitin sulfate
WU Ai-ping
ZHOU Hai-ying
CHEN Yin-ping
Li Qian
Bai Jing
Abstract：Objective To estimate the effects of different thawing methods on the histological structure and vitali -ty of cryopreserved homograft cardiac valves in liquid nitrogen with chondroitin sulfate ( CS) .Methods A total of 48 rab-bit aortic valves were randomly divided into control group and experiment groups (Ⅰ~Ⅲ, n=12 ) .All the valves were sterilized, after which the activity of endothelial cells in control group was examined and the valve tissues were observed under light and electron microscopes .In experiment groups , valves were cryopreserved for 1 month in liquid nitrogen con-tained CS by the same programmed cooling procedure , after which the valves in Group Ⅰ,ⅡandⅢwere thawed at rates of 100, 30, 15 ℃/min, respectively.The cellular activity and pathological observation were also performed .Results Compared with controls , significant morphologic changes and cellular activity impairment were revealed in both Group ⅠandⅡ(P<0.05);while there was no significant difference was observed in Group Ⅲ (P>0.05).Conclusion The thawing rate of 15℃/min is proven the best procedure in reserving the histological structure and cellular activity of cryopr -eserved valves .
Keywords：homograft cardiac valveschondroitin sulfatecryopreservationthawing methods
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 809-811 )