The effects of adipose-derived stem cells transplantation in full thickness cutaneous deficiency repairing in rats
SUN Chuan-wei
CHEN Hua-de
BIAN Hui-ning
ZHENG Shao-yi
LIU Zu-an
LAI Wen
Abstract：Objective To investigate the effects of adipose -derived stem cells ( ADSCs ) transplantation in full thickness cutaneous deficiency repairing in rats .Methods Full thickness cuntaneous deficiency models were constructed in 25 SD rats and randomly divided into control group , collagen membrane group ( negative control ) and ADSCs-collagen membrane group;in which allogeneic skin graft ( ASG) alone, ASG plus collagen membrane , and ASG plus ADSCs-col-lagen membrane were given for wound covering , respectively.The level of wound healing was observed .Tensile test, HE staining and in situ hybridization were carried out to assess the tensile strength , histological changes and ADSCs′expres-sion in new skin , respectively .Results Animal experiment showed that ADSCs and collagen membrane transplantations were safe.The wound healing rate of ADSCs -collagen membrane group [(94.24 ±3.13)%] was significantly higher than those of control group [(84.57 ±2.42)%] and collagen membrane group [(84.72 ±9.70)%].There was no sig-nificant difference was revealed in the tensile strength of new skin among the three groups ( P >0.05 ) .HE staining showed that there were neogenetic skin appendages in experimental group rather than controls .According to in situ hybrid-ization, SRY DNA was positive in the new skin of ADSCs -collagen membrane group .Conclusion ADSCs transplanta-tion can promote the full thickness cutaneous deficiency repairing in rats .
Keywords：adipose-derived stem cellsfull thickness cutaneous deficiencywound healing
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 805-808 )