Molecular cloning and sequence analysis of the human pancreatic kallikrein gene
Abstract：Objective To clone the human tissue kallikrein gene in Chinese. Methods Total RNA was extracted from human pancreas and human tissue kallikrein (KK) gene cDNA was amplified by PCR after reverse-transcription using Oligo(dT) primer. The original kallikrein cDNA was recovered and inserted into the Sma I site of KS plasmid. After restriction endonuclease digestion analysis, KK cDNA was sequenced by ABI 377 sequencer. Results The cloned kallikrein gene is about 832 bp in length. A sequence comparison of this with the reported KK sequence in the Genbank indicated that the cloned Chinese kallikrein gene has one nucleotide difference . The coded amino acid is Asp in the Genbank reported gene, while the coding amino acid of Chinese kallikrein gene is Asn. Conclusion The cloned kallikrein gene can be used for further study in gene therapy and other study.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：2( 291-292 )