Study on killing effect of retrovirus-mediated HSV-TK gene therapy for osteosarcoma
QIAO Hui
Qiu Jushi
Zhu Quansheng
Abstract：Objective To study the gene therapy for osteosarcoma in vitro.Methods Retroviral vector and HSV-TK/GCV system were used.Results Recombinant retroviral vector (DORHyTK) containing Hygromycin phosphotransferase-thymidine Kinase fusion gene(HyTK) was constructed and introduced into PA317 retrovirus packaging cell by DOTAP. After selective culturing and measuring of retroviral titers, the osteosarcoma cells OS732 and SAOS-2 were infected by pseudotype retroviral supernatant, which did not contain replication-competent retrovirus(RCR). The HSVTK gene existence and expression in OS732TK and SAOS-2TK cells were examined by PCR and RNA dot blot analysis. HSV-TK/GCV gene therapy system showed its strong killing effects in osteosarcoma cells OS732 and SAOS-2 by means of MTT colorimetric assay, with the former stronger than the latter. However the “bystander effect” was weeker in OS732 than in SAOS-2 and was stronger in the high-density population group. Conclusion HSV-TK/GCV gene therapy system may provide a new therapeutic approach for treatment of osteosarcoma.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 195-198 )