Amplification and cloning of human stem cell factor full-length cDNA by RT-PCR
Tan Yunnian
Tan Wenbin
PENG Xinhua
Abstract：Objective To study the expression and regulation of human stem cell factor in eukaryotic cell, its full-length cDNA was amplified by RT-PCR and its cloning vector was constructed. Methods HepG2 cells were cultured in RPMI1640 containing 10% bovine serum, and the cultured cells were harvested and their RNA was extracted;The 1.14 kb cDNA was amplified by RT-PCR, and the full-length cDNA fragment was inserted in the pGEM-T vector. Results The recombinant plasmid was cleaved with restrictive endonuclease and sequencing result showed that the cloning vector was successfully created. Conclusion The recombinant construct of the full-length cDNA provids available conditions for further study on expression and regulation of stem cell factor.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：2( 16-18 )