Detection of TT virus infection and genotypes in blood donors
Zhang Zhi
Su Hongmei
Zhang Zhen
Abstract：Objective To investigate TT virus infection and distribution of genotypes, and to build up an effective method for detecting TT virus infection in blood donor. Methods Nested polymerase chain reaction(nPCR), enzyme - linked immunosorbent assay (ELISA) and microporous plate hybridization methods were performed to detect TT virus DNA,anti- TT virus IgG, and TT virus genotypes. Results TT virus DNA and anti - TT virus IgG were detected in 40(14.9 %)and 24(9.0 % ) of 268 blood donors, The positive rates of TT virus DNA in remunerated regular and non - remunerated blood donors was 35.2 % (32/91 ) and 4.5 % (8/177), respectively, There was significant difference between the two groups (χ2=42.1, P<0.005) .The TT virus genotypes were genotype I in all 40 cases of TT virus DNA positives. Conclusion TT virus infection is common in the blood donors and a high prevalence of TT virus infection in remunerated regular blood donors has been demonstrated. It is necessary to strictly control the number of professional blood donors and encourage the practice of donating blood gratts. At present, nPCR is an effective method for detecting TT virus infection. Most of TT virus genotypes detected in blood donors are genotype I.
Keywords：Blood donor TT virus Genotype
Publication Date：2000-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：2( 1026-1027 )