The direct effect of circulating immune cell from idiopathic nephrotic children on glomerular intrinsic cells

doi:10.3969/j.issn.1001-9448.2000.12.002

Abstract：Objective To investigate the direct effect of circulating immune cell from idiopathic nephrolic children on glomerular epithelial cell and mesangial cell. Methods Twenty eight children with idiopathic nephropathy and 15 healthy children were randomly selected and divided into four groups. Group 1: the nephrotic children with no steroid treatment; Group 2: nephrotic children received prednison - treatment for at least 2 weeks; Group 3: prednison - treated children with no proteinuria; Group 4: healthy children. The peripheral blood mononuclear cell were collected from the children and their conditional medium (PBMC - CM) were prepared. Glomerular epithelial and mesangial cells culture, 3H - prolin and 3H - TdR incorporation, and competivitive combined radioimmunoassay were used to investigate:①The synthesis of laminin (LN), total collagen, collagen Ⅲ and collagen Ⅳ by cultured rat glomerular epithelial cell (GEC) after treatment with PBMC - CM of the children in 4 groups; ②The proliferation of glomerular mesangial cell (GMC). Results ①The inhibition coefficient of LN in these 4 groups were 0.95, 1.02, 1.01 and 1.03 respectively; the 3H - prolin incorporation coefficient of 4 groups were 0.93, 1.24, 1.23 and 1.11; the inhibition coefficient of collagen Ⅲ were 0.96, 1.00, 0.99 and 1.01. There were significant differences in the three coefficiencies of Group 1 compared with each of other groups (P<0.05), while the inhibition coefficient of collagen 1Ⅳ shown no significant difference among the 4 groups 1.04, 1.05, 1.04 and 1.08 respectively (P>0.05); ②The 3H- TdR incorporation coefficients of the GMC of 4 groups were 1.21, 1.53, 1.50 and 1.10 respectively, and shown no significant difference among the 4 groups ( P > 0.05). Conclusion Circulating immune cells from idiopatic nephrotic children have direct effect on the LN, collagen and collagen Ⅲ synthesis of rat GEC. The bioactivity can be neutrolized by prednison treatment, but the circulating immune cell has no effect on GMC proliferation.