Monitoring and Prevention Control of Nucleic Acid Contamination in Veterinary PCR Detection Laboratories
WEI Dawei
PENG Tao
HAN Kaiou
TANG Chao
XIONG Hailin
TANG Anwen
YI Chunhua
Abstract：PCR testing has become widely implemented in veterinary testing laboratories at all levels,yet minute nucleic acid contamination can readily compromise test results.This paper analyzes the primary sources of nucleic acid contamination and proposes an environmental surveillance protocol for veterinary laboratory PCR testing,covering sampling methods,detection techniques,monitoring frequency,and confirmation of results.Furthermore,it formulates comprehensive measures for preventing and controlling laboratory environmental contamination,including rational laboratory layout,establishment of a risk monitoring system,enhanced personnel training,appropriate placement of quality control materials,selection of optimal detection methods,and strict implementation of decontamination procedures.These integrated strategies aim to effectively control PCR contamination within veterinary laboratories,thereby reducing the occurrence of false-positive results.
Keywords：PCR detectionContaminationEnvironmental monitoringPrevention control
Publication Date：2026-02-18
Online Publishing Date：2026-03-20(First online date of this platform, not the publication date of the document)
Pages：5( 116-120 )