Isolation,Identification and Drug Sensitivity Test of a Trueperella pyogenes Strain from Vulpes zerda
PENG Shiming
WANG Guoqian
CHEN Xuanjiao
ZENG Fanwen
SA Jiaqi
Abstract：To identify the pathogenic bacteria causing the death of a Vulpes zerda from respiratory failure at a zoo in Guangdong Province,lung tissue samples were collected for bacterial isolation and purification,morphological microscopy,biochemical tests,antimicrobial susceptibility testing,16S rRNA gene sequencing,phylogenetic tree construction and virulence gene detection.Results showed that one strain of Trueperella pyogenes(a Gram-positive,short-rod-shaped bacterium)was isolated from the lung tissue.Biochemical tests indicated that the isolate was positive for lactose,maltose,and glucose fermentation,but negative for sucrose,rhamnose,and mannitol utilization.16S rRNA gene sequencing revealed more than 99%homology between the Vulpes zerda isolate and reference strains of T.pyogenes from swine and cattle,clustering within the same branch on the phylogenetic tree.Virulence gene detection confirmed the presence of at least six virulence genes:Plo-g2,Plo-g1,NanP,FimA,FimE,and FimC.Antimicrobial susceptibility testing demonstrated sensitivity to multiple antibiotics tested,with resistance observed only to two sulfonamide antibiotics(sulfamethoxazole and trimethoprim).This study enriches the characterization of Trueperella pyogenes derived from Vulpes zerda,providing a reference for research on wildlife pathogens.
Keywords：Vulpes zerdaTrueperella pyogenesDrug sensitivity test16S rRNA
Publication Date：2025-12-18
Online Publishing Date：2025-12-31(First online date of this platform, not the publication date of the document)
Pages：6( 106-111 )