Comparative Analysis of the Immune Effects of Recombinant Avian Influenza Virus(H5+H7)Trivalent Inactivated Vaccine on Specific Pathogen-Free-Chickens and Shelduck
DONG Jiawen
ZHANG Yanan
HUANG Yunzhen
CHEN Lei
KUANG Ruihuan
ZENG Xiaoduo
XIE Lexin
SUN Minhua
Abstract：To investigate the differences in immune effects of recombinant avian influenza virus(H5+H7)trivalent inactivated vaccine on poultry and waterfowl,this study immunized different groups of SPF chickens and shelduck with the recombinant avian influenza virus(H5+H7)trivalent inactivated vaccine at 14 days of age(primary immunization)and 42 days of age(secondary immunization),respectively.Blood samples were collected before and weekly after vaccination to detect avian influenza virus HI antibody titer against H5 Re-13、H5 Re-14 and H7 Re-4.Additionally,the expression levels of cytokine IL-2,IL-4,and IL-6 in the serum 14 days after the second immunization(56 days of age)were assessed.The results showed that all three commercially available H5+H7 trivalent inactivated vaccines could induce high HI antibody levels in SPF chickens within four weeks of primary immunization,with 100%qualification rates for H5-Re13,H5-Re14,and H7-Re4 antibodies.However,after primary immunization in shelduck,the qualification rate for H5-Re13 antibody was only 60%,with a low average titer,while the qualification rates for H5-Re14 and H7-Re4 antibodies were 60%-100%and 90%-100%,respectively.SPF chickens and shelduck could further increase HI antibody levels after booster immunization,but the antibody levels decreased four weeks after booster immunization.The immunogenicity of H5 subtype avian influenza virus inactivated vaccine in shelduck was much lower than that in chickens,while the H7 subtype avian influenza virus inactivated vaccine had high immunogenicity in both chickens and shelduck.The recombinant AIV trivalent inactivated vaccine did not induce the expression of cellular immune cytokines(IL-2 and IL-4)or significantly induce the expression of humoral immune cytokine IL-6 in SPF chickens and shelduck.There was no direct correlation between the the differences in HI antibody levels induced by the various vaccines and the expression levels of cytokines IL-2,IL-4,and IL-6.
Keywords：Avian influenza virusInactivated vaccineH5+H7SPF chickensshelduckimmune effects
Publication Date：2025-12-18
Online Publishing Date：2025-12-31(First online date of this platform, not the publication date of the document)
Pages：8( 61-68 )