Establishment of in Vitro Culture System of Spermatogonial Stem Cells Isolated from Testis Samples of Guangdong Small-spotted Pigs
XIN Haiyun
ZHOU Shimeng
MENG Fanming
Abstract：The study aimed to study the characterization of spermatogonial stem cells(SSCs)from Guangdong Small-spotted Pig testis in vitro after separation,purification and in vitro culture.The investigation employed enzyme digestion,density gradient centrifugation,and differential adhesion methods for cell enrichment,and cell immunofluorescence staining was used to assess separation efficiency,cell proliferation and differentiation.The results indicated that the combination of three separation methods effectively improved the purity of SSCs,which were successfully proliferated in vitro,forming distinct cell colonies by day 14,then cell colonies proliferation was significantly increased by day 21.Immunostaining showed that the SSCs maker,UCHL-1 was expressed highly within 28 days,while the expression of C-kit on day 56 indicated cell differentiation.
Keywords：Guangdong Small-spotted PigTestisSpermatogonial stem cellsImmunofluorescence stainingUCHL-1
Publication Date：2025-12-18
Online Publishing Date：2025-12-31(First online date of this platform, not the publication date of the document)
Pages：6( 49-54 )