Development of Indirect ELISA Method for Antibody Detection of Classical Swine Fever Virus
YAN Shiqi
LI Fukun
QI Dongmei
LAI Yuehui
HUANG Fuqiang
FENG Yuxin
ZHOU Xiaomin
FAN Quanbao
Abstract：Classical swine fever(CSF)caused by classical swine fever virus(CSFV),is a highly contagious and lethal viral disease in swine,for which vaccination remains a critical preventive strategy,antibody levels is an important indicator for evaluating the immune effect of immunized pig herds.In this study,the recombinant E2Y protein of CSFV was successfully expressed using a prokaryotic expression system.The purified E2Y protein served as a coating antigen,and an indirect ELISA method for detecting CSFV-specific antibodies was established through the systematic optimization of experimental conditions.Results indicated that the optimal coating concentration of the E2Y protein was 0.25 μg/mL.The assay demonstrated no cross-reactivity with positive sera against common swine viruses and exhibited high specificity,sensitivity,and reproducibility.The concordance between this method and a commercial ELISA kit reached 91.67%.This study successfully developed an indirect ELISA for CSFV antibody detection,providing a technical basis for further optimization and development of CSFV antibody detection reagents.
Keywords：Classical swine fever virusAntibody detectionIndirect ELISA method
Publication Date：2025-10-18
Online Publishing Date：2025-11-03(First online date of this platform, not the publication date of the document)
Pages：8( 66-72,120 )