Isolation and Identification of One Strain of Bovine Mannheimella Haemolytica
LI Jintao
WANG Jinping
AN Qingcong
YAO Jun
Abstract：To investigate the cause of the disease and death of Simmental beef cattle from a large-scale farm in Malong County,Yunnan Province,PCR tests were carried out on some bovine virus diseases(BVDV、BCV、BAdV-7、BAdV-3、BRSV and BEFV)and bacterial pathogens(MB and Pasteurella pultocida)in the lung tissues of the sick and dead cattle sent for testing,followed by bacteria isolation,purification,Gram stained,biochemical identification and antimicrobial susceptibility testing.The results showed that the nucleic acid and bacterial PCR test results of the tested bovine virus were negative.The isolated and purified bacteria were Gram-negative short rods,biochemically characterized as Mannheimella hemolytica.Antimicrobial susceptibility testing revealed sensitivity to polymyxin B and resistant to spectinomycin,chloramphenicol,cefoperazone,cefotaxime,minocycline,ceftriaxone,ceftazidime and cefuroxime.These findings provide theoretical reference for the effective diagnosis and treatment of"shipping fever"(bovine Mannheimella hemolytica infection,either single or mixed).
Keywords：CattleMannheimella hemolyticaSeparation and identificationAntimicrobial resistance
Publication Date：2025-10-18
Online Publishing Date：2025-11-03(First online date of this platform, not the publication date of the document)
Pages：6( 52-56,101 )