Establishment and Application of a Double Fluorescence Quantitative Polymerase Chain Reaction Detection Method for Novel Goose Astrovirus Types 1 and 2
XU Hang
LIU Mengfan
YIN Zhenghao
LU Yongkun
LI Yuze
LUO Haojian
LIN Junjie
WANG Yiqiao
LIU Yingying
MEI Kun
HUANG Shujian
Abstract：In order to develop a credible dual fluorescence quantitative polymerase chain reaction(qPCR)for detecting novel goose astrovirus type 1(NGAstV-1)and type 2(NGAstV-2),primers were designed to establish a dual fluorescence qPCR detection method using ORF2 protein of NGAstV-1 and NGAstV-2,and 36 samples of goose liver and kidney tissue suspected to be infected with NGAstV-1 and NGAstV-2 were evaluated in this study.The test results showed that the correlation coefficients of qPCR were 0.997 and were negative for novel duck reovirus(NDRV),avian influenza virus(AIV),fowl adenovirus(FAdV),and goose parvovirus(GPV).The minimum detection limit was 1.12×101 copies/μL.The reproducibility test results indicate good reproducibility,with a coefficient of variation between groups of less than 0.8%and a coefficient of variation within groups of less than 0.5%.It can be seen that the qPCR can efficiently and specifically detect NGAstV-1 and NGAstV-2,providing a feasible method for NGAstV monitoring.
Keywords：Novel goose astrovirusqPCRORF2
Publication Date：2025-08-18
Online Publishing Date：2025-08-26(First online date of this platform, not the publication date of the document)
Pages：11( 83-93 )