Research on the Inhibitory Effect of Rose Essential Oil and Active Ingredients on Three Aquatic-derived Bacteriaincluding Streptococcus Iniae
XU Yibing
LIN Zhiyu
FENG Kaien
LIANG Kaiqi
TUO Jiangyu
LI Jialing
LIU Siping
LIU xiaoxi
Abstract：To experimentally investigate the in vitro inhibitory zonesize and minimum inhibitory concentration of rose essential oil and its antimicrobial active ingredients citronellol,geraniol and nerol against three aquatic pathogens,namely Vibrio parahaemolyticus(V.parahaemolyticu),Streptococcus iniae(S.iniae),and Vibrio harveyi(V.harveyi).The inhibitory effects of rose essential oil and its antibacterial components(citronellol,geraniol and nerol)on three aquatic pathogenic bacteria(V.parahaemolyticu,S.iniae and V.harveyi)was tested.The diameter of the inhibition zones of rose essential oil and its antibacterial agents was determined using the methods of punch-hole and cross-validation.The minimum inhibitory concentrations(MIC)of rose essential oil and its antibacterial compounds were determined using the 96-well plate microdilution method.Finally,the fractional inhibitory concentration(FIC)indices of the three active ingredients were determined using the micro-broth chessboard cross-validation method.The results demonstrated that rose essential oil and its active ingredients exhibited antibacterial activity against V.parahaemolyticu,S.iniae and V.harveyi.Among these,citronellol demonstrated the most pronounced antibacterial effect.The three rose essential oil active ingredients were tested in combination with one another to evaluate their joint antibacterial effect,with the findings indicating that the combined effect was either irrelevant or antagonistic.Rose essential oil and its active ingredients have been demonstrated to exert notable bacteriostatic effects on aquatic pathogenic bacteria.However,the simultaneous application of its active ingredients in a clinical setting is not recommended.This study will provide a theoretical foundation for the future application of the antibacterial effects of rose essential oil in aquaculture.
Keywords：Rose essential oilAntibacterial effectCombined antibacterial effectVibrio parahaemolyticusStreptococcus iniaeVibrio harveyi
Publication Date：2025-08-18
Online Publishing Date：2025-08-26(First online date of this platform, not the publication date of the document)
Pages：6( 45-50 )