Surveillance and Analysis of Avian Leukosis in Milin Tibetan Chicken
XU Xinchun
HE Jingyi
YAN Xia
CHEN Peng
JIA Shaoyan
JI Jian
LUO Chenglong
Abstract：To explore the detection effect of different detection methods and the feasibility of the combination of avian leukemia virus(ALV)infection and AL purification,the study in chicken,using ALV p27 antigen ELISA method and ALV-JPCR nucleic acid detection method,from 2020 to 2024 a total of 1 333 randomly collected whole blood samples for ALV detection analysis.The results showed that in Tibetan chickens,the positive rate of ALV p27 antigen ELISA test was 15.42%,ALV-JPCR nucleic acid test was 2.49%.The total positive rate of the two detection methods was 17.23%,among which only 3 individuals were positive for the two detection methods,accounting for 3.95%of the total positive individuals.In addition,since AL purification in 2020 to 2024,the AL positive rate of milin Tibetan chickens decreased from 17.23%to 0 between 2020 and 2024.The results show that there are mixed infection of ALV-J subgroup and other subgroups in the flock;the combination of the two methods can greatly improve the ALV detection rate and can provide reference for the actual purification work;the AL purification of milin Tibetan chicken group is significant,which basically realizes the effective control of AL in the flock,which can provide data support for the AL prevention and control purification of this species.
Keywords：Milin Tibetan chickenAvian leukosisPCR nucleic acid testp27 antigen ELISA test
Publication Date：2025-08-18
Online Publishing Date：2025-08-26(First online date of this platform, not the publication date of the document)
Pages：5( 40-44 )