Improvement of the Purification and Detection Method of Chicken White Dysentery
CHEN Huihong
CHEN Peng
MO Zhixin
GUO Zhaowen
XIE Chunlin
WU Mingying
HE Jingyi
LUO Xuehui
LUO Chenglong
JI Jian
Abstract：To investigate the impact of cryopreservation conditions on the detection of antibodies against Salmonella pullorum and the feasibility of using residual plasma after avian leukosis detection for pullorum disease detection,this study selected 3 860 three-yellow chickens for pullorum disease testing in batches.The detection results using different anticoagulants,cryopreserved blood samples,serum,and residual plasma were compared with the standard on-site whole blood plate agglutination detection.The results showed that the positive detection rates of lithium heparin anticoagulated blood,ethylenediaminetetraacetic acid dipotassium salt(EDTA dipotassium salt,EDTA-2K)anticoagulated blood and residual plasma were highly consistent with the on-site whole blood plate agglutination detection results.The positive individuals detected by the on-site whole blood plate agglutination method could be completely detected in both cryopreserved and non-cryopreserved serum and residual plasma samples.The positive detection rate of serum samples was slightly higher than the standard method,and short-term cryopreservation had no significant adverse effect on the detection results.In addition,the actual application effect of using residual plasma for pullorum purification was evident.
Keywords：Chicken dysentery detectionPlasma residual fluidFrozen conditions
Publication Date：2025-06-18
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 105-110 )