Study on the Immune Regulation of Porcine Intestinal Epithelial Cells by Reuterin and its Potential Application in Piglet Health
WU Qi
YANG Xi
Abstract：The weaning stage of piglets is confronted with challenges such as imperfect intestinal development,unbalanced microbial community,and an immature immune system,resulting in frequent intestinal diseases and severely affecting growth performance.The search for alternatives to antibiotics is urgent.In this study,Lactobacillus reuteri LR1 strain was isolated from the feces of healthy weaned piglets,and its biological characteristics were analyzed,and the effect of its metabolite reuterin on porcine intestinal epithelial cells(IPEC-J2)was explored.The results showed that the LR1 strain grew best at 39.25℃and pH=6.387;reuterin had significant antibacterial activity against ETEC K88,and the inhibitory effect increased with the increase of concentration;it had a time-and concentration-dependent effect on the survival rate of IPEC-J2 cells,with cytotoxicity in the early stage and an increase in survival rate in the later stage;different concentrations of reuterin(2 μL/mL,20 μL/mL,50 μL/mL)induced the differential expression of interleukins and chemokines in IPEC-J2 cells.This study provides a theoretical basis for the application of reuterin as an antibiotic-free feed additive in piglet breeding and helps to promote the green and healthy development of the livestock industry.
Keywords：ReuterinPorcine intestinal epithelial cellsImmune regulationProbiotic metabolite
Publication Date：2025-06-18
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：10( 53-62 )