Genetic Evolution Analysis of Novel Duck Reovirus σC Protein and Preparation of Polyclonal Antibodies
ZHONG Richeng
LIN Yinsheng
ZHANG Jipei
Abstract：Novel duck reovirus(NDRV)is capable of inducing a contagious disease in waterfowl,including ducks,Beijing ducks,and geese,which is primarily characterized by irregular hemorrhaging and necrosis of the liver and spleen.In recent years,the incidence of this disease in waterfowl in Guangdong Province has been on the rise.In order to understand the genetic evolution characteristics of the novel duck enteritis virus in Guangdong Province,a strain of NDRV was isolated from affected Muscovy ducks in a duck farm in Foshan,Guangdong.Analysis of the σC gene sequence of this strain revealed that it belongs to the same branch as NDRV,with nucleotide similarity ranging from 95.1%to 99.6%.The genetic distance from typical MDRV and ARV was far,with similarities below 50%.Compared to the SH12 and DH13 strains,this strain showed variations in 15 amino acid residues.To understand the antigenicity of the σC protein of this strain,a pET32a-σC plasmid was further constructed,and a method for stable expression of recombinant σC protein in BL21(DE3)was established.Western blot analysis confirmed that this protein can specifically bind to NDRV-positive serum and rabbit polyclonal antibodies.This study provides a reference for further understanding the genetic evolution characteristics of NDRV in waterfowl in Guangdong Province and the development of gene-engineered vaccines.
Keywords：Novel duck enteritis virusσC proteinGenetic evolution analysisPolyclonal antibody preparation
Publication Date：2025-04-18
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 30-36 )