Effects of Fresh Fermented Rapeseed Meal on the Growth rate,Serum Biochemical Indexes,Hepatic Antioxidant Capacity,Intestinal Digestive Enzyme Activity and Intestinal Flora in Grass Carp
WANG Yilin
WANG Yajun
CHEN Jianchou
ZHU Xuezhi
SHI Anning
WANG Yue
LIAO Jingxuan
LIU Liyan
Abstract：The experiment was to study the effects of different levels of moist fermented rapeseed meal fermented by Bacillus subtilis on the growth,serum physiology and biochemistry,intestinal digestibility and intestinal flora of grass carp(Ctenopharyngodon idella).A total of 450 grass carp with an initial average weight of(51.60±0.56)g were randomly divided into 5 groups with 3 replicates and 30 fish per replicate.The amount of moist fermented rapeseed meal in each group was 0(control group),10%,20%,30%and 40%,respectively,and the amount of rapeseed meal was reduced accordingly.The experiment lasted for 8 weeks.The results showed that the condition factor of 20%and 40%moist fermented rapeseed meal groups was significantly higher than that of control group(P<0.05).The contents of total cholesterol and high density lipoprotein in 40%group were significantly higher than those in control group(P<0.05).Dietary supplementation of more than 20%of moist fermented rapeseed meal significantly increased intestinal α-amylase activity(P<0.05),and dietary supplementation of more than 30%of moist fermented rapeseed meal significantly increased intestinal trypsin activity(P<0.05).With the increase of the replacement level of moist fermented rapeseeds,the numbers of Firmicutes,verrucobacteria and actinobacteria in intestinal tract increased,while the numbers of Proteobacteria and chloromyces decreased.Under the experimental conditions,moist fermented rapeseed meal had a certain growth-promoting effect,which could improve intestinal digestive enzyme activity and intestinal flora composition.The feed prepared with 20%moist fermented rapeseed meal is more beneficial to increase the fatness of grass carp,increase the lipid metabolism capacity of liver,reduce the oxidative damage of liver and improve the intestinal flora.The feed prepared with 30%and 40%moist fermented rapeseed meal formula has a good effect on improving intestinal digestive enzymes of grass carp.
Keywords：Moist fermented rapeseed mealGrass carpFermented feedIntestinal floraSerum physiology biochemistry
Publication Date：2025-02-17
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：10( 33-42 )