Chitosan Alleviates LPS-induced Intestinal Barrier Damage by Regulating Tight Junction Proteins Via MAPK Signaling Pathway
XIE Huili
WU Lianyun
Ju Xianghong
LIU Xiaoxi
LI Youquan
LI Yin
GAO Yuan
ZHOU Qiu
TANG Ruifan
YONG Yanhong
Abstract：The aim of this experiment was to investigate the mechanism of action of chitosan(COS)in alleviating LPS-induced impairment of porcine intestinal epithelial cell(IPEC-J2)barrier function.The experiment was divided into four groups,namely,control group,LPS-treated group,COS-treated group,and COS+LPS-treated group.The proliferation rate of intestinal epithelial cells was measured by(1)CCK-8 method;(2)TEER method and FITC-dextran method for monolayer fusion epithelial permeability;(3)Western blot for cellular tight junction proteins ZO-1,Occludin,Claudin-1,and Claudin-4 and mitogen-activated protein kinase(MAPK)and mitogen-activated protein kinase(MAPK),ERK,JNK,P38 and their phosphorylated protein expression levels.The results showed that 1)the proliferation rate of IPEC-J2 was significantly decreased under LPS stimulation,and COS pretreatment significantly inhibited the LPS-induced decrease in the proliferation rate of IPEC-J2(P＜0.01).2)COS increased the resistivity of intestinal monolayer fusion epithelium and significantly ameliorated the LPS-induced decrease in intestinal monolayer fusion epithelium resistivity and the increase in permeability to FITC-dextran(P＜0.01).3)COS significantly inhibited the LPS-induced up-regulation of TNF-α and IL-β in IPEC-J2(P＜0.01).4)After LPS stimulation,cellular tight junction proteins were significantly down-regulated,while COS pretreatment significantly inhibited LPS-induced down-regulation of tight junction proteins ZO-1,Occludin,Claudin-1 and Claudin-4;COS pretreatment inhibited LPS-induced down-regulation of p-ERK expression.Thus,chitooligosaccharides have obvious anti-inflammatory and mucosal protective functions;the protection of chitooligosaccharides against inflammatory bowel disease is related to the up-regulation of tight junction proteins;chitooligosaccharides improve intestinal barrier damage by regulating tight junction proteins expression via MAPK signaling.
Keywords：ChitosanTight junction proteinMAPK
Publication Date：2024-12-18
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：9( 41-49 )