Identification of canine parvovirus and sequence analysis of VP2 gene in ornamental animals
PANG Zhuo
HUANG Xuezhe
ZUO Kejing
CHEN xuanjiao
LUO Jun
GUO Xiaofeng
HUANG Mian
Abstract：In order to confirm the diagnosis and understand the genetic evolution of ornamental animals suspected to be infected with canine parvovirus,such as nutria and caracal cats,genomic DNA was extracted from their anal swabs and feces.The VP2 gene was sequenced by PCR,and the genetic evolution tree was analyzed with Chinese and foreign reference strains.The results showed that 15 of the 15 samples were amplified with a specific band of about 756 bp,which was identified as CPV after comparison with the CU-4 strain of FPV standard,with a positive rate of 100%.The genetic evolution tree showed that the isolates had a close genetic relationship with CPV-2a and CPV-New2a subtypes isolated from Beijing and a relatively long genetic distance from other reference strains.The results of this study enrich the research data of canine parvus in ornamental animals and provide references for the genetics and prevention of the disease.
Keywords：Ornamental animalsCanine parvovirusVP2 geneGenetic analysis
Publication Date：2023-12-18
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 46-49 )