Effects of Ceruloplasmin on Maturation Rate and Embryonic Development of Porcine Oocytes Cultured in Vitro
JING Huansong
ZHOU Rong
LUO Lvhua
MAI Ranbiao
SU Qiaoyun
JI Hongmei
YU Wanxian
SHI Junsong
Abstract：The in vitro culture of porcine oocytes decreased the maturation rate and embryo development efficiency due to oxidative stress.Ceruloplasmin has antioxidant effects,but its role in mammalian oocyte maturation has been rarely studied.The aim of this study was to investigate the effects of ceruloplasmin as a novel antioxidant on the maturation rate of porcine oocytes and the development of parthenogenetic embryos in vitro.Cumulus oocyte complex was extracted from swine ovaries and cultured in vitro.Gradient concentrations of ceruloplasmin(0,1.25,2.5,5.0 and 10.0 μg/mL)were supplemented in the mature medium to calculate the maturation rate of oocytes and detect the reactive oxygen species(ROS)levels in oocytes.After the activation of parthenogenesis,the rate of cleavage,blastocyst rate and total cell counts of blastocysts were measured.Addition of 2.5 μg/mL ceruloplasminincreased the maturation rate of porcine oocytes and the blastocyst rate of parthenogenetic embryos(P<0.05),while decreased the intracellular ROS content.The addition of ceruloplasmin to porcine oocytes in vitro reduce the oxidative stress of oocytes,promote the maturation of oocytes in vitro and the development of parthenogenetic embryos.
Keywords：CeruloplasminReactive Oxygen SpeciesIn vitro culturePorcine oocyteEarly embryonic development
Publication Date：2023-12-18
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 28-33 )