Variation Analysis of the Nsp2 and GP5 Gene ofPRRSV (GD strain) under Antibody Selective Pressures
Abstract：PRRSV strain GD was continuously propagated in Marc-145 cells for 40 passages without PRRSV antibody or with PRRSV antibody and the viruses after passages were named RRRSV-GD-f40 or RRRSV-GDAb-f40 respectively. Nsp2 and GP5 genes were cloned and sequenced. Comparison results showed that the amplification fragment of Nsp2 gene of all isolates were 796 bp long and shared 98.0% or 97.9% similirities with maternal strain respectively. The GP5 genome analyses showed that their cDNAs of GP5 were 603bp long and shared 99.2% or 98.8% similirities with maternal strain respectively.
Keywords：PRRSVNsp2GP5Sequence analysis
Publication Date：2012-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 29-31,37 )