Amplification and Sequence Analysis of ITS and 5.8S rDNA of Ascarid Nematodes from Xiaocrhua Pig
Abstract：Our research objective was to identify ascarid samples isolated from small ear floral pig. The ascarid DNA in small ear floral pig was extracted.The internal transcribed spacer (ITS) and 5.8S of ribosomal DNA (rDNA) of two ascarid samples were amplified by PCR using a pair of conserved primers.The lengths of ITS sequence were both 1441 bp long. The two ascarid specimens had identical ITS and 5.8S rDNA sequences.The sequences were blasted in GenBank and the similarities was 88%, 98%, 86% or below 90% with the sequences of Baylisascaris transfuga, Ascaris suum, Ascaris lumbricoides or other ascarid.
Keywords：AscaridsPCR amplificationInternal transcribed spacers(ITS)Sequence analysis
Publication Date：2012-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 37-39 )