Optimization of the Cultrue Technology of the Haemophilus ParagaUinarum (strain Hpg-8) and the Identification of the Immunogenicity
Abstract：In this present study different amount of yeast extract, chicken serum and NADH were used as the additives for the semisynthesis culture medium to prepare strain Hpg-8. The cultivation result of each experiment group was evaluated by comparing the viable count and the OD600 value of the culture, and the immunogenicity of the culture were evaluated by the challenge protection test. Considering on the antigen concentration, the unit production costs, and the immunogenicity of each group, the optimal amount of the yeast extract, chicken serum and NADH was determined to be 10%, 5% or 0.0015% respectively. Using this cultivating condition, the viable count could reach 2.04 billion and the immunogenicity of the culture conformed with the requirements of the Veterinary Pharmacopoeia of the People's Republic of China.
Keywords：HaemophilusparagallinarumCultureOptimizationImmunogenicity
Publication Date：2012-01-01
Pages：4( 34-36,39 )