The Detection of Quinolone Residue in Tilapia Mossambica using ELISA and LC-MS/MS
Abstract：The quinolones residue in tilapia was detected by the methods of enzyme-linked immunosorbent assay(ELISA) and liquid chromatography-tandem mass spectrometry(LC-MS/MS). The results showed that in the ELISA test the recovery rate was from 73.4% to 89.5%, the coefficient of variation(CV)% was from 5.6% to 14.3%, the detection time was within 45 minutes and in the LC-MS/MS test the recovery rate was from 77.1% to 85.7%, the CV% was from 5.5% to 11.2%. The results of LC-MS/MS was more accurate and the CV% of LC-MS/MS was more small than the results of ELISA, but the test of LC-MS/MS was more expensive. So the ELISA test was suitable for the rapid screening of the quinolones residue in tilapia and the LC-MS/MS was only suitable for confirmation.
Keywords：QuinolonesResidueELISALC-MS/MS
Publication Date：2012-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 29-33 )