Studies on residues of Ofloxacin in black-bone silky fowl
Abstract：A reversed-phase high performance liquid chromatographic method has been developed for the determination of ofloxacin residues in black-bone silky fowl. After being extracted with phosphate buffer, ofloxacin was analyzed by HPLC （Hypersil BDS-C18） and detected by FLD （hex = 280 nm, hem = 450 nm） . The mobile phase was acetonitrile-phosphoric acid/triethylamine （18 ： 82）. The linear range was10-2 000p g/kg .The average recovery of ofloxacin was 86.3%, 83.2% or 82.5% in muscle, liver or kindney respectively .The minimal detectable concentration of ofloxacin was 10 ng/g. Results of this experiment showed that the depletion of ofloxacin in muscle and kidney was slow. After 40 days of withdrawal, the concentration of ofloxacin in the chickens used at 2 days old were 146.8μg/kg , 20.6μg/kg or 437.4μg/kg in muscle, liver and kidney respectively, The concentration of ofloxacin in the chickens used at 28 days old were 193.5μg/kg, 45.0μg/kg or 425.5μg/kg respectively. After 71 days of withdrawal, the concentration of ofloxacin in the chickens used at 2 days old were 11.2μg/kg or 12μg/kg in the muscle and liver respectively. This experiment showed that Ofloxacin should only be applied to the chickens younger than 10 days old with more than 70 days of withdrawal or should not be used in raising.
Keywords：OfloxacinBlack-bone silky fowlResidues
Publication Date：2012-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 33-36 )