The outer membrane protein type of five dominant Eschedchia coli serotypes in pigs in Guangdong area
Abstract：After the Escherichia coli strains were serotyped, it was found that five serotypes 09, 026, O60, O101 and O107 were the dominant serotypes. The outer membrane protein of eight strains from these five serotypes were extracted by ultrasonic cracking, N- twelve alkyl sodium sarcosinate treatment and ultracentrifugation. The method of SDS-PAGE electrophoresis was used for OMP separation. The results showed that there were three types in 8 strains（type I to type Ⅲ） . The OMP number of three strains from 026, O101 and O107 was one and it was named type I. The OMP number of four strains from O60 O101 and O107Twas two and it was named type II. The OMP number of one strain from 09 were three and it was named type Ⅲ. The results showed that the OMP types of serotypes 09, 026, 060. O101 and Ol07were different. The OMP types of the same serotype could be different and the OMP types of different serotypes mac be the same.
Keywords：Pig Escherichia coliSerotype OThe outer membrane proteinSubtyping
Publication Date：2012-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 21-23 )