Development and application of DPV UL35 gene PCR assay
Abstract：The PCR assay for the detection of Duck plague virus （DPV） was developed and was applied to detect the isolated strains and the artificial infected samples. According to the sequences ofDPV UL35 gene available in GenBank （GenBank： EF643558）, a pair of primers were designed and synthesized for the detection of DPV. PCR method for the rapid detection of DPV was developed by optimizing some reaction factors. The results showed that a 354 bp specific fragment could be amplified from the DPV DNA and the sensitivity of PCR was 470 ng DPV-DNA. The negative results were detected from other viruses including AIV-H9, MDRV, NDV, MPV and GPV. The positive rate of PCR method for detecting viral pathogens in 3 field isolates and 6 samples of the liver and spleen tissues of ducklings which were artificially infected by DPV BL8 strain was 100%. The results indicated that this PCR method was sensitive and specific for detecting DPV and could be used for DPV clinical diagnosis and epidemiology investigation.
Keywords：Duck plague virusPCRDetection
Publication Date：2012-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 33-36 )