Development of the fluorescent quantitative PCR method for detecting Pseudorabies virus wild strains
Abstract：According to the sequences of gE gene of pseudorabies virus（PRV）,one pair of primers and one Taqman probe were designed and synthesized for differentiating the wild strains of pseudorabies virus from the field samples by using LightCycle 480 real-time PCR machine.The detectable linear range of this novel qPCR method was from 1.0×102 to 1.0×1010 copies with the lowest detectable DNA limit of 4 copies.This rapid and sensitive qPCR test could be run in one hour to acquire the results.Its specificity by detecting 13 wild strains of pseudorabies viruses isolated in our laboratory was confirmed in which no any positive amplification for swine parvovirus and duck plague virus（duck enteritis virus） were produced.This method was more highly rapid,sensitive,specific and repeatable than the classical virus isolation and culture methods,and could be used for the detection of wild type of PRV in the field samples.
Keywords：Wild-type pseudorabies virusfluorescent quantitative PCRclinical detection
Publication Date：2012-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 31-34 )