Isolation, identification and 16S rRNA gene analysis of Pseudomonas aeruginosa strain GDC 10 isolated from the chicken
Abstract：Abstract. In this experiment a bacteria strain isolated fi＇om one dead chicken was identified as Pseudomonas aeruginosa by morphological characteristics, cultural characteristics and biological characterization. The 16s rRNA gene sequence of this bacteria was also obtained by PCR with a pair of common primers. This strain was closely related to the strains of Pseudomonas aeruginosa after the 16s rRNA sequence of this strain was blasted in GenBank. The nucleotide sequence homology was from 99% to 100% between this strain and reference Pseudomonas aeruginosa strains. The discrepancy was about 9% between this strain and E.coli., Salmonella or Pasteurella strains. The discrepancy was more than 18% between this strain and Riemerella strains. These results showed that sequencing of 16S rRNA gene was a useful method for identifing the bacteria.
Keywords：Pseudomonasaeruginosaisolationandidentification16SrRNAgenessequence analysis
Publication Date：2011-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 32-35 )