Development and preliminary application of fluorescence quantitative RT- PCR for detection of classical swine fever virus
Abstract：A set of primers and a TaqMan probe directed to the 5＇ NTR of CSFV genome sequences were designed for the PCR detection of CSFV. The total RNA extracted from the virus was used as the template for reverse transcription and PCR. The PCR products were cloned into pMD 18-T vector and the plasmid isolated from the positive clone was used for the standard material for the standard curve. So the specific RT-PCR method for detection of CSFV was established. The sensitivity of the assay was 10~ copies/tJ L. The viruses were detected in blood, spleen, lymph nodes and tonsils of the pigs immunized with different doses of classical swine fever cell vaccine.
Keywords：Classical swine fever virusFluorogenic quantitative PCRClassical swine fever cell vaccineVirusdistribution
Publication Date：2011-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 30-33 )