Application of a novel multifunctional nanoprobes modified by cancer cell membrane in breast cancer
QIN Hengyi
GAO Yujing
CUI Ting
DING Qimei
WANG Jiawei
JI Shuhan
TAO Jin
WEI Junhui
WANG Tingting
YANG Caixia
LI Xianglin
Abstract：Objective To develop a novel multifunctional integrated diagnostic and therapeutic nanoprobes targeting breast cancer,MnO2@DOX@Cancer Cell Membrane(MDC),evaluate its basic properties,and explore its magnetic resonance imaging(MRI)capabilities and chemotherapy effects.Methods MnO2 with a favorable morphology was synthesized.The cell membrane of 4T1 mouse breast cancer cells was obtained using chemical lysis and ultrasonic fragmentation techniques.A lipid extrusion method was used to synthesize the cell membrane-modified nanoprobes MDC loaded with doxorubicin(DOX).Key properties of MDC,including morphology,surface potential,and particle size,were characterized,and its MRI capability was explored.The cytotoxic effects of MDC at different concentrations on tumor cells were observed to assess its inhibitory effect on breast cancer tumors in chemotherapy.The integrated diagnostic and therapeutic functions at the cellular level were also verified.Results The nanoprobes MDC with a nanoflower-like structure were successfully synthesized,with a Zeta potential of-20±1.5 mV and an average particle size of 151.2 nm.Transmission electron microscopy(TEM)imaging confirmed successful coating of the cell membrane onto the nanoprobes.MRI results showed that MDC exhibited excellent MRI T1 imaging ability in a simulated tumor microenvironment.Laser confocal microscopy experiments further confirmed that MDC had excellent homologous targeting capability.Cytotoxicity experiments indicated that MDC significantly killed cancer cells.Conclusion This study successfully synthesized a multifunctional integrated diagnostic and therapeutic nanoprobes MDC,which exhibited excellent homologous targeting and anti-tumor effects in chemotherapy,significantly enhanced MRI imaging,and demonstrated integrated diagnostic and therapeutic functions at the cellular level.
Keywords：cancer cell membranenanoprobebreast cancerchemotherapy therapymagnetic resonance imaging
Publication Date：2025-05-20
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 529-534 )