Metagenomic identification and recombination analysis of mixed infection with diverse lineages of PRRSV-2 on a large-scale pig farm in Guangdong
CHEN Xiuqiao
GUO Yajing
YAN Guangzhi
CHEN Shengnan
LIU Mingjie
HUANG Liangzong
Abstract：To investigate the genomic characterization of porcine reproductive and respiratory syndrome virus(PRRSV)in large-scale pig farms,viral metagenomics technology was applied to perform high-throughput sequencing of a lung tissue sample that was positive for PRRSV by RT-qPCR,and the sequencing results were subjected to virome analysis and genomic characterization.Two complete genome sequences of PRRSV were obtained from a lung tissue sample designated as GD202301 and GD202302,respectively.Genomic characterization showed that the complete genome sequence and ORF5 gene of GD202301 belonged to PRRSV-2 lineage 8 and lineage 3 in the genetic evolution analysis,respectively,and was produced by triple recombination of three strains,NADC30-like,JXA1-like and QYYZ-like.The complete genome sequence and the ORF5 gene of GD202302,both of which belonged to PRRSV-2 lineage 1 in the genetic evolutionary analysis,was triple recombined from three strains,NADC30-like,JXA1-like,and QYYZ-like.The presence of two types of PRRSV in a sample was found by viral metagenomics technology,which indicates that there is a mixed infection of two different spectrums of PRRSV in this farm,and provides data support for the understanding of PRRSV infection in this farm.
Keywords：viral metagenomics technologyPRRSVcomplete genomegenetic evolutionary analysisviral recombination
Publication Date：2025-11-30
Online Publishing Date：2025-12-12(First online date of this platform, not the publication date of the document)
Pages：8( 81-88 )