Determination of vitamin A acetate in feed of hyperuricemia chicken model
CEN Zhi-fang
LIU Gui-yan
CHEN Li-chun
GUAN Zhen-cheng
LI Gui-qi
MENG Bi-fei
Abstract：Objective To establish a determination method for vitamin A in feed of hyperuricemia chicken model by HPLC.Method Using the eighteen alkyl silane bonded silica KromasilC18 column (250 mm * 4.6 mm, 5 μm); a mixture of methanol and water solution (97∶3) as the mobile phase, the flow rate was 1.0 mL·min-1;detection the wavelength was 325 nm; column temperature was at room temperature.Results The linear relationship of vitamin A in the range of 40-800 μg·mL-1is good and r=0.999 9, the average rate of recovery was 101.5%, RSD=2.43% Conclusion The method has the advantages of simple and rapid operation, accurate and stable operation and good reproducibility, and can be used as a determination method of vitamin A in mixed chicken feed.
Keywords：feed of hyperuricemia chicken modelvitamin Acontent determinationHPLC
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 64-68 )