Detection of prostate-specific antigen in patients with prostatic cancers by electrochemical luminescence

WU Zhi-peng; TAN Yi-jing; LIU Fang; YAN Ai-fen; LIU Jing; HUANG Wen-zhu; TANG Dong-sheng

doi:10.3969/j.issn.1008-0171.2014.06.008

Abstract：Objective:To investigate the value of detection of prostate-specific antigen T-PSA, F-PSA and F-PSA/T-PSA in the diagnosis and differential diagnosis of prostate cancer and prostatic hyperplasia. Methods:The blood samples of 86 prostate cancer patients, 487 prostatic hyperplasia patients and 188 healthy persons from Foshan First People's Hospital were collected. The serum concentration of total prostate-specific antigen (T-PSA) and free prostate specific antigen (F-PSA)were examined by electrochemical luminescence. The F-PSA/T-PSA ratio was calculated. The significant difference of T-PSA, F-PSA and F-PSA/T-PSA ratio among the prostate cancer group, prostatic hyperplasia group and control group were calculated statistically and respectively. Results:The T-PSA, F-PSA, F-PSA/T-PSA ratio in the prostatic hyperplasia group and prostate cancer group was significantly different from those of the control group (p<0.01). The T-PSA, F-PSA, F-PSA/T-PSA ratio in the prostate cancer group was significantly different from those of the prostatic hyperplasia group (p<0.01). When the concentration of T-PSA ranged from 4.0 ng/ml to 10.0 ng/ml, there was no significant difference in the T-PSA between the prostate cancer group and the prostatic hyperplasia group, while the F-PSA and F-PSA/T-PSA ratio were significantly different between the two groups, When the concentration of T-PSA ranged from 4.0 ng/ml to 10.0 ng/ml, the F-PSA/T-PSA threshold take 0.1, 0.15, 0.16, 0.17, 0.2, respectively, the sensitivity was 33.3%, 61.9%, 61.9%, 69.0%, 76.2 %, respectively; the specificity was 89.1% , 70.1% , 64.7% , 60.0% , 44.6% , respectively. The critical value (0.15)was the best screening indicator. Conclusions:T-PSA can be used as an important screening indicator of prostate cancer. When concentration of T-PSA ranged from 4.0 to 10.0ng/ml, the F-PSA/T-PSA ratio was a better choice for prostate cancer screening than T-PSA, in which a higher sensitivity and specificity were achieved when the F-PSA/T-PSA threshold was 0.15.