Studies on soluble expression of the four repeats of cholecystokinin-33 and its application
Abstract：Four repeats of chicken CCK-33（abbr.4CCK33） gene were successfully amplified by PCR from the pRSETA-4CCK33 recombinant plasmid using the primers designed according to sequences of pRSETA plasmid.The four repeats of CCK-33 gene were inserted into plasmid pBCX to obtain recombinant plasmid in which four repeats of CCK33 gene were fused into.The pBCX-4CCK33 was conducted into E.coli BL-21 to induce four repeats of CCK-33 gene fusion expression.The fusion protein band with molecular weight of 53 000 was detected on the SDS-PAGE gel and most fusion proteins were soluble.Western-blot showed that the expression products can specifically reacted with the antisera of CCK-8.The soluble form was purified.The purified fusion proteins were used as antigen to prepare oil-emulsion vaccines,and the pigs were immunized.Average daily gain in pigs immunized with 0.5 mg/mL and 1.5 mg/mL 4CCK33 vaccine were significantly increased by 5.28% and 5.92% respectively,while that of the feed intake increased by 3.03% and 2.53%,compared with the control group.
Keywords：cholecystokinin-33high soluble expressionimmunizationgrowth performance
Publication Date：2012-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 62-66 )