DNA content detection of microcystis aeruginosa PCC 7806 on different N/P nutrients by FCM
Abstract：In the formation of algae bloom,the proportion of nitrogen and phosphorus is the major influential factor.The optimal N/P for alage is 16：1.It is assumed that the particular proportion be related to the growth and division of cyanobacteria cells.The most vital role in vivo of phosphorus is involved in DNA、RNA synthesis.As for nitrogen,it is related to the protein synthesis.The appropriate ratio between N and P may be presumed as a reflection of the relation in quantity between the growth and division of alage cell.In order to verify the assumption,we used the technology of FCM to detect the cells of M.aeruginosa stained by PI which were cultured on different N/P nutrients and set up four groups of different N/P ratios,16∶1,160∶1,1∶1 and lacking of NP.It was found that when N/P=16∶1,the DNA content of M.aeruginosa cells is higher than others.We concluded that the division of these cells is at an optimal level.
Keywords：flow cytometry（FCM）N/PDNA content detection
Publication Date：2011-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 47-51 )