Construction of recombinant plasmid pPICZαC/Pocine 17Serine-interferon-beta
Abstract：A pair of primer was designed according to Porcine interferon beta gene sequence recorded in GenBank.According to codon bias of yeast,the 3rd（TAT）,7th（CGA）and 164th（CGG） codon was mutated to（TAC）,（AGA）,（CGT）respectively.And the 17th cysteine（TGT） was mutated to Serine（AGT）.The IFN-β Ser17 which was amplified with PCR technique was cloned into expression vector pPICZαC.The results indicated that the IFN-β Ser17 mutant was obtained through PCR technique and the pPICZαC-IFN-β Ser17 recombinant expression vector was successfully constructed.
Keywords：porcine interferon betagene site-directed mutagenesisgene cloningrecombinant plasmid
Publication Date：2011-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 44-46,75 )