Optimization of Fermentation Process of Agaricus blazei Mycelium Based on Orthogonal Design
ZOU Yan-hui
HUANG Zhen-liang
LIN Yong
ZHU Hong-yang
LI Yong-ning
Abstract：By taking Agaricus blazei mycelium FJWZY-1 as the starting strain and its biomass as the evaluation index,the three main factors affecting the fermentation(including medium pH,shaker oscillation speed,and carbon-to-nitrogen ratio)were studied.Based on the single-factor experiments,a three-factor,three-level orthogonal optimization experiment was designed to determine the fermentation conditions for Agaricus blazei mycelium.The results showed that:the data from the single-factor experiments indicated that the optimal pH,shaker oscillation speed,and carbon-to-nitrogen ratio(C/N ratio)of the medium for the fermentation of Agaricus blazei mycelium FJWZY-1 were 6.0,100 r/min,and 10∶1,respectively,and the biomass of Agaricus blazei mycelium FJWZY-1 was 6.6 g·L-1.On this basis,the response surface methodology(RSM)was used to optimize the fermentation process.The results demonstrated that the optimal fermentation conditions were consistent with those from the single-factor experiment,and the order of the influencing power of different factors was as follows:carbon-to-nitrogen ratio(C/N ratio)of the medium＞pH＞shaker oscillation speed.The study laid a foundation for realizing the rapid growth of Agaricus blazei mycelium,increasing its biomass,further extracting Agaricus blazei polysaccharides,and realizing its large-scale fermentation production and cultivation.
Keywords：Agaricus blazeiFermentation processOrthogonal experimentPolysaccharide
Publication Date：2025-12-28
Online Publishing Date：2026-03-11(First online date of this platform, not the publication date of the document)
Pages：4( 54-57 )