Optimization of Tissue Culture Propagation Technology for Cattleya Protocorms

ZHU Wei-yin

doi:10.13651/j.cnki.fjnykj.2025.11.005

Abstract：Through the analysis of the factors affecting the proliferation of Cattleya protocorm tissue culture technology,a set of efficient tissue culture and rapid propagation technology system was discussed to realize the efficient breeding of Cattleya excellent varieties.In this study,the protocorms of 10 different Cattleya hybrid combinations were used as the materials to explore the effects of hybrid combinations,medium additives,plant growth regulators,medium types and other conditions on the proliferation of Cattleya protocorms.The results showed that under the same culture conditions,there were significant differences in the proliferation coefficient of protocorm of the ten different hybrid combinations,except for ZX-2 and ZX-3.ZX-6 had the highest proliferation coefficient of 5.83.The further study by using ZX-6 original bulbs as the material found that the optimal combination and concentration of additives were 3 g·L-1 peptone and 0.5 g·L-1 Hyponex No.1,under which the proliferation coefficient of ZX-6 protocorm reached 4.34.The impact of plant growth regulators on the proliferation of Cattleya protocorms varied,ranked in the order of influence as 6-BA＞NAA＞KT,with both 6-BA and NAA having extremely significant effects on the proliferation of protocorm(P＜0.01).The most suitable hormone combination for ZX-6 protocorm proliferation was A2B2C1,that is,6-BA 0.5 mg·L-1+NAA 0.2 mg·L-1+KT 0.25 mg·L-1.Different basal media significantly affected the proliferation of protocorm.Appropriately reducing the mass concentration of inorganic salts in the medium while adding a certain concentration of sucrose and activated carbon could promote the proliferation of Cattleya protocorms.The best proliferation effect for ZX-6 protocorms was achieved when the basal medium,sucrose concentration,and activated carbon concentration were 1/2 MS,25 g·L-1,and 0.5 g·L-1 respectively.The experiment showed that the most suitable proliferation medium for ZX-6 hybrid combination was 1/2 MS supplemented with 6-BA 0.5 mg·L-1,NAA 0.2 mg·L-1,KT 0.25 mg·L-1,peptone 3 g·L-1,Hyponex No.1 0.5 g·L-1,sucrose 25 g·L-1,and activated carbon 0.5 g·L-1.